Romidepsin

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Romidepsin (FK228，depsipeptide) 是一种有效的 HDAC1 和 HDAC2 抑制剂，IC50 分别为 36 nM 和 47 nM。

Romidepsin (FK228, depsipeptide) is a potent HDAC1 and HDAC2 inhibitor with IC50 of 36 nM and 47 nM, respectively.(In Vitro):Romidepsin (0-72 hours; 0-80 nM) inhibits proliferation of HCC cells in dose-dependent manner.Romidepsin (0-48 hours; 0-60 nM) leads to a time- and dose-dependent induction of cell cycle arrest in the G2/M phase in HCC cells.Romidepsin (0-48 hours; 0-60 nM) promotesapoptosis in HCC cells, increases c-caspase-3, c-caspase-9, and c-PARP protein expression.(In Vivo):Romidepsin (intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days) inhibited the tumor growth, reveals a higher expression of p-cdc25C, ki67, c-caspase-3 and c-PARP, and a lower expression of Ki-67 in Romidepsin treated tumors .

Cell Proliferation Assay Cell Line:HCC cells Concentration:0 nM; 10 nM; 20 nM; 30 nM; 40 nM; 50 nM; 60 nM; 70 nM; 80 nM Incubation Time:0 hours; 12 hours; 24 hours; 48 hours; 72 hours Result:Inhibited HCC cells proliferation.Cell Cycle Analysis Cell Line:HCC cells Concentration:0 nM;15 nM; 30 nM; 60 nM Incubation Time:12 hours;24 hours; 48 hours Result:Caused a G2/M arrest.Western Blot Analysis Cell Line:HCC cells Concentration:0 nM;15 nM; 30 nM; 60 nM Incubation Time:12 hours;24 hours; 48 hours Result:Increaesd c-caspase-3, c-caspase-9, and c-PARP expression in HCC cells.

Animal Model:Nude mice with Huh7 cells Dosage:0.5 and 1 mg/kg Administration:Intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days Result:Suppressed tumor growth in mouse xenograft models.

FK228, Depsipeptide

Cell Cycle/DNA Damage

HDAC

HDAC1| HDAC2

Cancer

——

128517-07-7

540.7

C24H36N4O6S2

>98% (HPLC)

DMSO: 10 mg/mL (18.49 mM)

O=C([C@H](C(C)C)NC(/C(N1)=C/C)=O)O[C@](CC(N[C@H](C(C)C)C(N2)=O)=O)([H])/C=C/CCSSC[C@]2([H])C1=O

(1S,4S,7Z,10S,16E,21R)-7-ethylidene-4,21-bis(1methylethyl)-2-oxa-12,13-dithia-5,8,20,23-tetraazabicyclo[8.7.6]tricos-16ene-3,6,9,19,22-pentone

(-20℃)

With Ice Pack

≥ 2 years